Global Methylomic and Transcriptomic Analyses Reveal the Broad Participation of DNA Methylation in Daily Gene Expression Regulation of Populus trichocarpa.
Identifieur interne : 000924 ( Main/Exploration ); précédent : 000923; suivant : 000925Global Methylomic and Transcriptomic Analyses Reveal the Broad Participation of DNA Methylation in Daily Gene Expression Regulation of Populus trichocarpa.
Auteurs : Lixiong Liang [République populaire de Chine] ; Yingying Chang [République populaire de Chine] ; Junqian Lu [République populaire de Chine] ; Xiaojuan Wu [République populaire de Chine] ; Qi Liu [République populaire de Chine] ; Weixi Zhang [République populaire de Chine] ; Xiaohua Su [République populaire de Chine] ; Bingyu Zhang [République populaire de Chine]Source :
- Frontiers in plant science [ 1664-462X ] ; 2019.
Abstract
Changes in DNA methylation patterns in different tissues, at various developmental stages, and under environmental stimuli have been investigated in plants. However, the involvement of DNA methylation in daily gene expression regulation and the plant circadian clock have not been reported. Here, we investigated DNA methylomes and mRNA transcriptomes from leaves of P. trichocarpa over 24 h by high-throughput sequencing. We found that approximately 15.63-19.50% of the genomic cytosine positions were methylated in mature poplar leaves, with approximately half being in the form of asymmetric CHH sites. Repetitive sequences and transposable elements (TEs) were heavily methylated, and the hAT and CMC-EnSpm transposons were more heavily methylated than other TEs. High methylation levels were observed upstream and downstream of the transcribed region, medium in exon and intron, low in untranslated region (5'-UTR and 3'-UTR) of genic regions. In total, about 53,689 differentially methylated regions (DMRs) were identified and CHH context was the most abundant type among daily DNA methylation changes. The DMRs overlapped with over one third of the total poplar genes, including plant defense genes. In addition, a positive correlation between expression levels and DNA methylation levels in the gene body region were observed in DMR overlapping genes. About 1,895 circadian regulated genes overlapped with DMRs, with 871 hypermethylated genes with down-regulated expression levels and 881 hypomethylated genes with up-regulated expression levels, indicating the possible regulation of DNA methylation on the daily rhythmic expression of these genes. But rhythmic DNA methylation changes were not detected in any oscillator component genes controlling the plant circadian clock. Our results suggest that DNA methylation participates widely in daily gene expression regulation, but is not the main mechanism modulating the plant circadian clock.
DOI: 10.3389/fpls.2019.00243
PubMed: 30873202
PubMed Central: PMC6403135
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Global Methylomic and Transcriptomic Analyses Reveal the Broad Participation of DNA Methylation in Daily Gene Expression Regulation of <i>Populus trichocarpa</i>.</title><author><name sortKey="Liang, Lixiong" sort="Liang, Lixiong" uniqKey="Liang L" first="Lixiong" last="Liang">Lixiong Liang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chang, Yingying" sort="Chang, Yingying" uniqKey="Chang Y" first="Yingying" last="Chang">Yingying Chang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lu, Junqian" sort="Lu, Junqian" uniqKey="Lu J" first="Junqian" last="Lu">Junqian Lu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wu, Xiaojuan" sort="Wu, Xiaojuan" uniqKey="Wu X" first="Xiaojuan" last="Wu">Xiaojuan Wu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Qi" sort="Liu, Qi" uniqKey="Liu Q" first="Qi" last="Liu">Qi Liu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Weixi" sort="Zhang, Weixi" uniqKey="Zhang W" first="Weixi" last="Zhang">Weixi Zhang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Su, Xiaohua" sort="Su, Xiaohua" uniqKey="Su X" first="Xiaohua" last="Su">Xiaohua Su</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Bingyu" sort="Zhang, Bingyu" uniqKey="Zhang B" first="Bingyu" last="Zhang">Bingyu Zhang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30873202</idno><idno type="pmid">30873202</idno><idno type="doi">10.3389/fpls.2019.00243</idno><idno type="pmc">PMC6403135</idno><idno type="wicri:Area/Main/Corpus">000991</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000991</idno><idno type="wicri:Area/Main/Curation">000991</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000991</idno><idno type="wicri:Area/Main/Exploration">000991</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Global Methylomic and Transcriptomic Analyses Reveal the Broad Participation of DNA Methylation in Daily Gene Expression Regulation of <i>Populus trichocarpa</i>.</title><author><name sortKey="Liang, Lixiong" sort="Liang, Lixiong" uniqKey="Liang L" first="Lixiong" last="Liang">Lixiong Liang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chang, Yingying" sort="Chang, Yingying" uniqKey="Chang Y" first="Yingying" last="Chang">Yingying Chang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lu, Junqian" sort="Lu, Junqian" uniqKey="Lu J" first="Junqian" last="Lu">Junqian Lu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wu, Xiaojuan" sort="Wu, Xiaojuan" uniqKey="Wu X" first="Xiaojuan" last="Wu">Xiaojuan Wu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Qi" sort="Liu, Qi" uniqKey="Liu Q" first="Qi" last="Liu">Qi Liu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Weixi" sort="Zhang, Weixi" uniqKey="Zhang W" first="Weixi" last="Zhang">Weixi Zhang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Su, Xiaohua" sort="Su, Xiaohua" uniqKey="Su X" first="Xiaohua" last="Su">Xiaohua Su</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Bingyu" sort="Zhang, Bingyu" uniqKey="Zhang B" first="Bingyu" last="Zhang">Bingyu Zhang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Changes in DNA methylation patterns in different tissues, at various developmental stages, and under environmental stimuli have been investigated in plants. However, the involvement of DNA methylation in daily gene expression regulation and the plant circadian clock have not been reported. Here, we investigated DNA methylomes and mRNA transcriptomes from leaves of <i>P. trichocarpa</i> over 24 h by high-throughput sequencing. We found that approximately 15.63-19.50% of the genomic cytosine positions were methylated in mature poplar leaves, with approximately half being in the form of asymmetric CHH sites. Repetitive sequences and transposable elements (TEs) were heavily methylated, and the hAT and CMC-EnSpm transposons were more heavily methylated than other TEs. High methylation levels were observed upstream and downstream of the transcribed region, medium in exon and intron, low in untranslated region (5'-UTR and 3'-UTR) of genic regions. In total, about 53,689 differentially methylated regions (DMRs) were identified and CHH context was the most abundant type among daily DNA methylation changes. The DMRs overlapped with over one third of the total poplar genes, including plant defense genes. In addition, a positive correlation between expression levels and DNA methylation levels in the gene body region were observed in DMR overlapping genes. About 1,895 circadian regulated genes overlapped with DMRs, with 871 hypermethylated genes with down-regulated expression levels and 881 hypomethylated genes with up-regulated expression levels, indicating the possible regulation of DNA methylation on the daily rhythmic expression of these genes. But rhythmic DNA methylation changes were not detected in any oscillator component genes controlling the plant circadian clock. Our results suggest that DNA methylation participates widely in daily gene expression regulation, but is not the main mechanism modulating the plant circadian clock.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30873202</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Global Methylomic and Transcriptomic Analyses Reveal the Broad Participation of DNA Methylation in Daily Gene Expression Regulation of <i>Populus trichocarpa</i>.</ArticleTitle><Pagination><MedlinePgn>243</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2019.00243</ELocationID><Abstract><AbstractText>Changes in DNA methylation patterns in different tissues, at various developmental stages, and under environmental stimuli have been investigated in plants. However, the involvement of DNA methylation in daily gene expression regulation and the plant circadian clock have not been reported. Here, we investigated DNA methylomes and mRNA transcriptomes from leaves of <i>P. trichocarpa</i> over 24 h by high-throughput sequencing. We found that approximately 15.63-19.50% of the genomic cytosine positions were methylated in mature poplar leaves, with approximately half being in the form of asymmetric CHH sites. Repetitive sequences and transposable elements (TEs) were heavily methylated, and the hAT and CMC-EnSpm transposons were more heavily methylated than other TEs. High methylation levels were observed upstream and downstream of the transcribed region, medium in exon and intron, low in untranslated region (5'-UTR and 3'-UTR) of genic regions. In total, about 53,689 differentially methylated regions (DMRs) were identified and CHH context was the most abundant type among daily DNA methylation changes. The DMRs overlapped with over one third of the total poplar genes, including plant defense genes. In addition, a positive correlation between expression levels and DNA methylation levels in the gene body region were observed in DMR overlapping genes. About 1,895 circadian regulated genes overlapped with DMRs, with 871 hypermethylated genes with down-regulated expression levels and 881 hypomethylated genes with up-regulated expression levels, indicating the possible regulation of DNA methylation on the daily rhythmic expression of these genes. But rhythmic DNA methylation changes were not detected in any oscillator component genes controlling the plant circadian clock. Our results suggest that DNA methylation participates widely in daily gene expression regulation, but is not the main mechanism modulating the plant circadian clock.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Lixiong</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Yingying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Junqian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xiaojuan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Qi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Weixi</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Su</LastName><ForeName>Xiaohua</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Bingyu</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>02</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">DMRs</Keyword><Keyword MajorTopicYN="N">DNA methylation</Keyword><Keyword MajorTopicYN="N">Populus L.</Keyword><Keyword MajorTopicYN="N">circadian clock</Keyword><Keyword MajorTopicYN="N">daily gene expression</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>09</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>02</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>3</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>3</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>3</Month><Day>16</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30873202</ArticleId><ArticleId IdType="doi">10.3389/fpls.2019.00243</ArticleId><ArticleId IdType="pmc">PMC6403135</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Science. 2000 Dec 15;290(5499):2110-3</Citation><ArticleIdList><ArticleId IdType="pubmed">11118138</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2003 Aug;54(389):1879-87</Citation><ArticleIdList><ArticleId IdType="pubmed">12837818</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2005 Jul;6(7):544-56</Citation><ArticleIdList><ArticleId IdType="pubmed">15951747</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11796-801</Citation><ArticleIdList><ArticleId IdType="pubmed">16864782</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2006 Sep 22;126(6):1189-201</Citation><ArticleIdList><ArticleId IdType="pubmed">16949657</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Genet. 2006;40:409-48</Citation><ArticleIdList><ArticleId IdType="pubmed">17094740</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6752-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17409185</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2008 Mar 13;452(7184):215-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18278030</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Mar 13;323(5920):1481-5</Citation><ArticleIdList><ArticleId IdType="pubmed">19286557</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2009;60:357-77</Citation><ArticleIdList><ArticleId IdType="pubmed">19575587</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Genet. 2009;43:143-66</Citation><ArticleIdList><ArticleId IdType="pubmed">19659441</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2009 Jul 10;10(7):3168-85</Citation><ArticleIdList><ArticleId IdType="pubmed">19742130</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Environ. 2010 Apr;33(4):481-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19781008</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2010 Mar;11(3):204-20</Citation><ArticleIdList><ArticleId IdType="pubmed">20142834</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 May 11;107(19):8689-94</Citation><ArticleIdList><ArticleId IdType="pubmed">20395551</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Environ. 2010 Nov;33(11):1820-7</Citation><ArticleIdList><ArticleId IdType="pubmed">20545886</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21623-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21115819</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2011 Feb;155(2):906-15</Citation><ArticleIdList><ArticleId IdType="pubmed">21139085</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2011 Apr 1;27(7):1023-5</Citation><ArticleIdList><ArticleId IdType="pubmed">21296749</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2011 Jun;14(3):267-74</Citation><ArticleIdList><ArticleId IdType="pubmed">21450514</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2011 Jun 1;27(11):1571-2</Citation><ArticleIdList><ArticleId IdType="pubmed">21493656</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2011 Jul;62(11):3727-35</Citation><ArticleIdList><ArticleId IdType="pubmed">21633082</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(6):e16907</Citation><ArticleIdList><ArticleId IdType="pubmed">21694767</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12521-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21746919</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Evol. 2012 Jan;29(1):219-27</Citation><ArticleIdList><ArticleId IdType="pubmed">21813466</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(10):e26968</Citation><ArticleIdList><ArticleId IdType="pubmed">22066021</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2012 Jan 17;13:27</Citation><ArticleIdList><ArticleId IdType="pubmed">22251412</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2012 May;5(3):554-65</Citation><ArticleIdList><ArticleId IdType="pubmed">22379122</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2012 Mar;24(3):875-92</Citation><ArticleIdList><ArticleId IdType="pubmed">22438023</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2012 May;53(5):809-16</Citation><ArticleIdList><ArticleId IdType="pubmed">22492232</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Aug 7;109(32):E2183-91</Citation><ArticleIdList><ArticleId IdType="pubmed">22733782</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2012 Jul 02;13:300</Citation><ArticleIdList><ArticleId IdType="pubmed">22747568</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cells. 2012 Sep;34(3):279-87</Citation><ArticleIdList><ArticleId IdType="pubmed">22878891</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2012 Sep 28;151(1):194-205</Citation><ArticleIdList><ArticleId IdType="pubmed">23000270</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21540-5</Citation><ArticleIdList><ArticleId IdType="pubmed">23236129</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2013 Feb;31(2):154-9</Citation><ArticleIdList><ArticleId IdType="pubmed">23354102</ArticleId></ArticleIdList></Reference><Reference><Citation>Semin Cell Dev Biol. 2013 May;24(5):399-406</Citation><ArticleIdList><ArticleId IdType="pubmed">23499867</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2013 Aug 9;341(6146):1237905</Citation><ArticleIdList><ArticleId IdType="pubmed">23828890</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biol (Stuttg). 2014 Mar;16(2):419-27</Citation><ArticleIdList><ArticleId IdType="pubmed">23889779</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Cell Biol. 2014 Feb;24(2):90-9</Citation><ArticleIdList><ArticleId IdType="pubmed">23916625</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Genet. 2013;47:187-208</Citation><ArticleIdList><ArticleId IdType="pubmed">24016190</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Mol Biol. 2014 Jan;21(1):64-72</Citation><ArticleIdList><ArticleId IdType="pubmed">24336224</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2014 Jun;65(11):2895-913</Citation><ArticleIdList><ArticleId IdType="pubmed">24520020</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Neurosci. 2014 Mar;17(3):377-82</Citation><ArticleIdList><ArticleId IdType="pubmed">24531307</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2014 May;19(5):320-6</Citation><ArticleIdList><ArticleId IdType="pubmed">24618094</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2014 Oct;21:43-50</Citation><ArticleIdList><ArticleId IdType="pubmed">25026538</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2014 Aug;11(8):817-820</Citation><ArticleIdList><ArticleId IdType="pubmed">25042786</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2015 Feb;87:102-8</Citation><ArticleIdList><ArticleId IdType="pubmed">25576840</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Feb 24;10(2):e0118669</Citation><ArticleIdList><ArticleId IdType="pubmed">25710777</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2015 May 06;6:308</Citation><ArticleIdList><ArticleId IdType="pubmed">25999972</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2015 Aug;168(4):1417-32</Citation><ArticleIdList><ArticleId IdType="pubmed">26145151</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Endocrinol Metab. 2015 Sep;100(9):E1255-61</Citation><ArticleIdList><ArticleId IdType="pubmed">26168277</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Aug 13;10(8):e0135485</Citation><ArticleIdList><ArticleId IdType="pubmed">26270551</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2015 Oct 05;15:237</Citation><ArticleIdList><ArticleId IdType="pubmed">26438149</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2015 Nov 3;112(44):13729-34</Citation><ArticleIdList><ArticleId IdType="pubmed">26483493</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2016 Mar;67(7):2081-92</Citation><ArticleIdList><ArticleId IdType="pubmed">26944635</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2016 May 27;474(2):271-276</Citation><ArticleIdList><ArticleId IdType="pubmed">27086112</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2016 Jul 11;12(7):e1006141</Citation><ArticleIdList><ArticleId IdType="pubmed">27398721</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta Gene Regul Mech. 2017 Jan;1860(1):106-122</Citation><ArticleIdList><ArticleId IdType="pubmed">27487458</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2017 Jan;22(1):53-65</Citation><ArticleIdList><ArticleId IdType="pubmed">27666516</ArticleId></ArticleIdList></Reference><Reference><Citation>Alzheimers Dement. 2017 Jun;13(6):689-700</Citation><ArticleIdList><ArticleId IdType="pubmed">27883893</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2017 Jul 3;45(W1):W122-W129</Citation><ArticleIdList><ArticleId IdType="pubmed">28472432</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2018 Feb;16(2):672-687</Citation><ArticleIdList><ArticleId IdType="pubmed">28796917</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2017 Sep 25;7(1):12263</Citation><ArticleIdList><ArticleId IdType="pubmed">28947812</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2018 Feb;23(2):140-150</Citation><ArticleIdList><ArticleId IdType="pubmed">29074233</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E1069-E1074</Citation><ArticleIdList><ArticleId IdType="pubmed">29339507</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2018 Jan 19;13(1):e0191492</Citation><ArticleIdList><ArticleId IdType="pubmed">29352281</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2018 Apr;23(4):324-336</Citation><ArticleIdList><ArticleId IdType="pubmed">29395832</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Liang, Lixiong" sort="Liang, Lixiong" uniqKey="Liang L" first="Lixiong" last="Liang">Lixiong Liang</name></noRegion><name sortKey="Chang, Yingying" sort="Chang, Yingying" uniqKey="Chang Y" first="Yingying" last="Chang">Yingying Chang</name><name sortKey="Chang, Yingying" sort="Chang, Yingying" uniqKey="Chang Y" first="Yingying" last="Chang">Yingying Chang</name><name sortKey="Liang, Lixiong" sort="Liang, Lixiong" uniqKey="Liang L" first="Lixiong" last="Liang">Lixiong Liang</name><name sortKey="Liu, Qi" sort="Liu, Qi" uniqKey="Liu Q" first="Qi" last="Liu">Qi Liu</name><name sortKey="Liu, Qi" sort="Liu, Qi" uniqKey="Liu Q" first="Qi" last="Liu">Qi Liu</name><name sortKey="Lu, Junqian" sort="Lu, Junqian" uniqKey="Lu J" first="Junqian" last="Lu">Junqian Lu</name><name sortKey="Lu, Junqian" sort="Lu, Junqian" uniqKey="Lu J" first="Junqian" last="Lu">Junqian Lu</name><name sortKey="Su, Xiaohua" sort="Su, Xiaohua" uniqKey="Su X" first="Xiaohua" last="Su">Xiaohua Su</name><name sortKey="Su, Xiaohua" sort="Su, Xiaohua" uniqKey="Su X" first="Xiaohua" last="Su">Xiaohua Su</name><name sortKey="Wu, Xiaojuan" sort="Wu, Xiaojuan" uniqKey="Wu X" first="Xiaojuan" last="Wu">Xiaojuan Wu</name><name sortKey="Wu, Xiaojuan" sort="Wu, Xiaojuan" uniqKey="Wu X" first="Xiaojuan" last="Wu">Xiaojuan Wu</name><name sortKey="Zhang, Bingyu" sort="Zhang, Bingyu" uniqKey="Zhang B" first="Bingyu" last="Zhang">Bingyu Zhang</name><name sortKey="Zhang, Bingyu" sort="Zhang, Bingyu" uniqKey="Zhang B" first="Bingyu" last="Zhang">Bingyu Zhang</name><name sortKey="Zhang, Weixi" sort="Zhang, Weixi" uniqKey="Zhang W" first="Weixi" last="Zhang">Weixi Zhang</name><name sortKey="Zhang, Weixi" sort="Zhang, Weixi" uniqKey="Zhang W" first="Weixi" last="Zhang">Weixi Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000924 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000924 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30873202
|texte= Global Methylomic and Transcriptomic Analyses Reveal the Broad Participation of DNA Methylation in Daily Gene Expression Regulation of Populus trichocarpa.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30873202" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |